Publications: Dr. Sanford Bernstein's Lab Personnel Home Page

Neal CL, Kronert WA, Camillo JRT, Suggs JA, Huxford T, Bernstein SI. (2024) Aging-affiliated post-translational modifications of skeletal muscle myosin affect biochemical properties, myofibril structure, muscle function, and proteostasis. Aging Cell. Mar 20:e14134

Kronert WA, Hsu KH, Madan A, Sarsoza F, Cammarato A, Bernstein SI. (2022) Myosin Transducer Inter-Strand Communication Is Critical for Normal ATPase Activity and Myofibril Structure Biology (Basel). Jul 29;11(8):1137.

Trujillo AS, Hsu KH, Viswanathan MC, Cammarato A, Bernstein SI. (2022) The R369 Myosin Residue within Loop 4 Is Critical for Actin Binding and Muscle Function in Drosophila. International Journal of Molecular Sciences. 2022; 23(5):2533. https://doi.org/10.3390/ijms23052533

Adriana S. Trujillo, Karen H. Hsu, Joy Puthawala, Meera C. Viswanathan, Amy Loya, Thomas C. Irving, Anthony Cammarato, Douglas M. Swank, and Sanford I. Bernstein. (2021) Myosin dilated cardiomyopathy mutation S532P disrupts actomyosin interactions, leading to altered muscle kinetics, reduced locomotion, and cardiac dilation in Drosophila. Mol Biol Cell. 2021 Aug 19;32(18):1690-1706.

Kaylyn M Bell, Alice Huang, William A Kronert, Sanford I Bernstein, Douglas M Swank. (2021) Prolonged myosin binding increases muscle stiffness in Drosophila models of Freeman-Sheldon syndrome. Biophys J. 2021 Mar 2;120(5):844-854.

Marieke J Bloemink, Karen H Hsu, Michael A Geeves, Sanford I Bernstein. (2020) Alternative N-terminal regions of Drosophila myosin heavy chain II regulate communication of the purine binding loop with the essential light chain. J Biol Chem. 2020 Oct 16;295(42):14522-14535.

Yiming Guo, William A Kronert, Karen H Hsu, Alice Huang, Floyd Sarsoza, Kaylyn M Bell, Jennifer A Suggs, Douglas M Swank, Sanford I Bernstein. (2020) Drosophila myosin mutants model the disparate severity of type 1 and type 2B distal arthrogryposis and indicate an enhanced actin affinity mechanism. Skelet Muscle. 2020 Aug 15;10(1):24.

Caldwell JT, Mermelstein DJ, Walker RC, Bernstein SI, Huxford T. (2020) X-ray Crystallographic and Molecular Dynamic Analyses of Drosophila melanogaster Embryonic Muscle Myosin Define Domains Responsible for Isoform-Specific Properties. J Mol Biol. 2020 Jan 17;432(2):427-447.

Bell KM, Kronert WA, Huang A1, Bernstein SI, Swank DM. (2019) The R249Q hypertrophic cardiomyopathy myosin mutation decreases contractility in Drosophila by impeding force production. J Physiol. 2019 May;597(9):2403-2420.

Gaida E, Barrios AJ, Wolkowicz R, Crowe SE, Bernstein SI, Quintana Serrano MA, Dumbauld JN, Pakiz B, Cripps RM, Arredondo EM, Martinez ME, Madanat H. (2019) Educating the Next Generation of Undergraduate URM Cancer Scientists: Results and Lessons Learned from a Cancer Research Partnership Scholar Program. J Cancer Educ. 2019 Nov 14.

Rao DS, Kronert WA, Guo Y, Hsu KH, Sarsoza F, Bernstein SI (2019) Reductions in ATPase activity, actin sliding velocity and myofibril stability yield muscle dysfunction in Drosophila models of myosin-based Freeman Sheldon syndrome. Mol Biol Cell. Jan 1; 30(1): 30-41. Journal Cover Image

Chandran S, Suggs JA, Wang BJ, Han A, Bhide S, Cryderman DE, Moore SA, Bernstein SI, Wallrath LL, Melkani GC. (2019) Suppression of myopathic lamin mutations by muscle-specific activation of AMPK and modulation of downstream signaling. Hum Mol Genet. 2019 Feb 1;28(3):351-371.

Kronert WA, Bell KM, Viswanathan MC, Melkani GC, Trujillo AS, Huang A, Melkani A, Cammarato A, Swank DM, Bernstein SI. (2018) Prolonged cross-bridge binding triggers muscle dysfunction in a Drosophila model of myosin-based hypertrophic cardiomyopathy. Elife. 2018 Aug 13;7

Lee KH, Sulbarán G, Yang S, Mun JY, Alamo L, Pinto A, Sato O, Ikebe M, Liu X, Korn ED, Sarsoza F, Bernstein SI, Padrón R, Craig R. (2018) Interacting-heads motif has been conserved as a mechanism of myosin II inhibition since before the origin of animals. Proc. Natl. Acad. Sci. U.S.A. Feb 27;115(9):E1991-E2000

Viswanathan MC, Tham RC, Kronert WA, Sarsoza F, Trujillo AS, Cammarato A, Bernstein SI. (2017) Myosin storage myopathy mutations yield defective myosin filament assembly in vitro and disrupted myofibrillar structure and function in vivo. Hum Mol Genet. Dec 15;26(24):4799-4813. Journal Cover Image

Melkani GC, Bhide S, Han A, Vyas J, Livelo C, Bodmer R, Bernstein SI. (2017) TRiC/CCT chaperonins are essential for maintaining myofibril organization, cardiac physiological rhythm, and lifespan. FEBS Lett. Nov;591(21):3447-3458.

Suggs JA, Melkani GC, Glasheen BM, Detor MM, Melkani A, Marsan NP, Swank DM, Bernstein SI. (2017) A Drosophila model of dominant inclusion body myopathy type 3 shows diminished myosin kinetics that reduce muscle power and yield myofibrillar defects. Dis Model Mech. Jun 1;10(6):761-771.

Cannon L, Zambon AC, Cammarato A, Zhang Z, Vogler G, Munoz M, Taylor E, Cartry J, Bernstein SI, Melov S, Bodmer R. (2017) Expression patterns of cardiac aging in Drosophila. Aging Cell. Feb;16(1):82-92.

Kooij V, Viswanathan MC, Lee DI, Rainer PP, Schmidt W, Kronert WA, Harding SE, Kass DA, Bernstein SI, Van Eyk JE, Cammarato A. (2016) Profilin modulates sarcomeric organization and mediates cardiomyocyte hypertrophy. Cardiovasc Res. 110(2):238-48.

Achal M, Trujillo AS, Melkani GC, Farman GP, Ocorr K, Viswanathan MC, Kaushik G, Newhard CS, Glasheen BM, Melkani A1, Suggs JA, Moore JR, Swank DM, Bodmer R, Cammarato A, Bernstein SI. (2016) A Restrictive Cardiomyopathy Mutation in an Invariant Proline at the Myosin Head/Rod Junction Enhances Head Flexibility and Function, Yielding Muscle Defects in Drosophila. J Mol Biol. 428(11):2446-61.

Kronert WA, Melkani GC, Melkani A, Bernstein SI. (2015) Failure to Communicate MYOSIN RESIDUES INVOLVED IN HYPERTROPHIC CARDIOMYOPATHY AFFECT INTERDOMAIN INTERACTION. J Biol Chem. 290: 29270–29280.

Bloemink MJ, Melkani GC, Bernstein SI, Geeves MA. (2016) The relay-converter interface influences hydrolysis of ATP by skeletal muscle myosin II. J. Biol. Chem.. 291:1763-1773.

Kaushik G, Spenlehauer A, Sessions AO, Trujillo AS, Fuhrmann A, Fu Z, Venkatraman V, Pohl D, Tuler J, Wang M, Lakatta EG, Ocorr K, Bodmer R, Bernstein SI, Van Eyk JE, Cammarato A, Engler AJ. (2015) Vinculin network–mediated cytoskeletal remodeling regulates contractile function in the aging heart. Sci Transl Med. 7(292):292ra99 Journal Cover Image

Kronert WA, Melkani GC, Melkani A, Bernstein SI. (2014) Mapping interactions between myosin relay and converter domains that power muscle function. J Biol Chem. 289: 12779-90.

Trujillo AS, Ramos R, Bodmer R, Bernstein SI, Ocorr K, Melkani GC. (2014) Drosophila as a potential model to ameliorate mutant Huntington-mediated cardiac amyloidosis. Rare Dis. 2: e968003.

Iwamoto H, Trombitás K, Yagi N, Suggs JA, Bernstein SI. (2014) X-ray diffraction from flight muscle with a headless myosin mutation: implications for interpreting reflection patterns. Front Physiol. 5: 416.

Lee CF, Melkani GC, Bernstein SI. (2014) The UNC-45 myosin chaperone: from worms to flies to vertebrates. Int Rev Cell Mol Biol. 313: 103-44.

Smith DA, Carland CR, Guo Y, Bernstein SI. (2014) Getting folded: chaperone proteins in muscle development, maintenance and disease. Anat Rec (Hoboken). Anat Rec. 297: 1637-49.

Melkani GC, Trujillo AS, Ramos R, Bodmer R, Bernstein SI, Ocorr K. (2013) Huntington's disease induced cardiac amyloidosis is reversed by modulating protein folding and oxidative stress pathways in the Drosophila heart. PLoS Genet. 10: e1004024 Journal Cover Image

Xie , H. B., A. Cammarato, N. S. Rajasekaran, H. Zhang, J. A. Suggs, H.-C. Lin, S. I. Bernstein, I. J. Benjamin and K. G. Golic (2013) The NADPH metabolic network regulates human alpha B-crystallin cardiomyopathy and reductive stress in Drosophila melanogaster. PLoS Genet. 9: e1003544

Kronert WA, Melkani GC, Melkani A, Bernstein SI. (2012) Alternative relay and converter domains tune native muscle myosin isoform function in Drosophila. J Mol Biol. 416:543-557.

Wang Y, Melkani GC, Suggs JA, Melkani A, Kronert WA, Cammarato A, Bernstein SI. (2012) Expression of the inclusion body myopathy 3 mutation in Drosophila depresses myosin function and stability and recapitulates muscle inclusions and weakness. Mol Biol Cell. 23:2057-65.

Kaushik G, Zambon AC, Fuhrmann A, Bernstein SI, Bodmer R, Engler AJ, Cammarato A. (2012) Measuring passive myocardial stiffness in Drosophila melanogaster to investigate diastolic dysfunction. J Cell Mol Med. 16:1656-1662.

Caldwell JT, Melkani GC, Huxford T, Bernstein SI. (2011) Transgenic expression and purification of myosin isoforms using the Drosophila melanogaster indirect flight muscle system. Methods. 2012 56:25-32. Journal Cover Image

Cammarato A, Li XE, Reedy MC, Lee CF, Lehman W, Bernstein SI. (2011) Structural basis for myopathic defects engendered by alterations in the myosin rod. J Mol Biol. 414:477-84.

Ramanath S, Wang Q, Bernstein SI, Swank DM. (2011) Disrupting the myosin converter-relay interface impairs Drosophila indirect flight muscle performance. Biophys J. 2011 10:1114-22.

Bloemink M.J., Melkani G.C., Dambacher C.M., Bernstein S.I., Geeves M.A. (2011) Two Drosophila myosin transducer mutants with distinct cardiomyopathies have divergent ADP and actin affinities. J Biol Chem. 286: 28435-28443.

Melkani, G. C., R. Bodmer, K. Ocorr and S.I. Bernstein (2011) The UNC-45 chaperone is critical for establishing myosin-based myofibrillar organization and cardiac contractility in the Drosophila heart model. PLoS One 6: e22579.

Cammarato, A., C. H. Ahrens, N. N. Alayari, E. Qeli, J. Rucker, M. C. Reedy, C. M. Zmasek, M. Gucek, R. N. Cole, J. E. Van Eyk, R. Bodmer, B. O'Rourke, S. I. Bernstein and D. B. Foster (2011) A mighty small heart: the cardiac proteome of adult Drosophila melanogaster. PLoS One 6: e18497.

Lee, C. F., A. V. Hauenstein, J. K. Fleming, W. C. Gasper, V. Engelke, B. Sankaran, S. I. Bernstein and T. Huxford (2011) X-ray crystal structure of the UCS domain-containing UNC-45 myosin chaperone from Drosophila melanogaster. Structure 19: 397-408.

Lee, C. F., G. C. Melkani, Q. Yu, J. A. Suggs, W. A. Kronert, Y. Suzuki, L. Hipolito, M. G. Price, H. F. Epstein and S. I. Bernstein (2011) Drosophila UNC-45 accumulates in embryonic blastoderm and in muscles and is essential for muscle myosin stability. J. Cell Sci. 24: 699-705.

Kronert, W. A., G. C. Melkani, A. Melkani and S. I. Bernstein (2010) Mutating the converter-relay interface of Drosophila myosin perturbs ATPase activity, actin motility, myofibril stability and flight ability. J. Mol. Biol. 398: 625-632.

Melkani, G. C., C. F. Lee, A. Cammarato and S. I. Bernstein (2010) Drosophila UNC-45 prevents heat-induced aggregation of skeletal muscle myosin and facilitates refolding of citrate synthase. Biochem. Biophys. Res. Comm. 396: 317-322.

Ocorr K., M. Fink, A. Cammarato, S. I. Bernstein and R. Bodmer. (2009) Semi-automated Optical Heartbeat Analysis of small hearts. J. Vis. Exp. 31: 1435.

Miller, M. S., C. M. Dambacher, A. F. Knowles, J. M. Braddock, G. P. Farman, T. C. Irving, D. M. Swank, S. I. Bernstein and D. W. Maughan. (2009) Alternative S2 hinge regions of the myosin rod affect myofibrillar structure and myosin kinetics. Biophys J. 96:4132-4143.

Bloemink M. J., C. M. Dambacher, A. F. Knowles , G. C. Melkani, M. A. Geeves and S. I. Bernstein. (2009) Alternative exon 9-encoded relay domains affect more than one communication pathway in the Drosophila myosin head. J Mol Biol. 2009 389:707-721.

Kronert, W. A., C. M. Dambacher, A. F. Knowles, D. M. Swank and S. I. Bernstein. (2008) Alternative relay domains of Drosophila melanogaster myosin differentially affect ATPase activity, in vitro motility, myofibril structure and muscle function. J. Mol. Biol. 379:443-456.

Littlefield, K. P., J. S. Chappie, A. B. Ward, M. K. Reedy, S. I. Bernstein, R. A. Milligan and M. C. Reedy. (2008) Similarities and differences between frozen-hydrated, rigor acto-S1 complexes of insect flight and chicken skeletal muscles. J. Mol. Biol. 381:519-528.

Cammarato, A., C. M. Dambacher, A. F. Knowles, W. A. Kronert, R. Bodmer, K. Ocorr and S. I. Bernstein. (2008) Myosin transducer mutations differentially affect motor function, myofibril structure and the performance of skeletal and cardiac muscles. Mol. Biol. Cell 19:553-562. Journal Cover Image

Yang, C., S. Ramanath, W. A. Kronert, S. I. Bernstein, D. W. Maughan, D. M. Swank. (2008) Alternative versions of the myosin relay domain differentially respond to load to influence Drosophila muscle kinetics. Biophys. J. 95:5228-5237.

Bernstein, S. I. and M. A. Geeves (2008) The XXXVII European muscle conference: Oxford September 2008. J. Muscle Res. Cell Motil. 29:253-256.

Miller, B. M., M. J., Bloemink, M. Nyitrai, S. I. Bernstein and M. A. Geeves. (2007) A variable domain near the ATP binding site in Drosophila muscle myosin is part of the communication pathway between the nucleotide and actin-binding sites. J. Mol. Biol. 368:1051-1066.

Suggs, J. A., A. Cammarato, W. A. Kronert, M. Nikkhoy, C. M. Dambacher, A. Megighian and S. I. Bernstein. (2007) Alternative S2 hinge regions of the myosin rod differentially affect muscle function, myofibril dimensions and myosin tail length. J. Mol. Biol. 367: 1312-1329.

Hess, N. K., P. A. Singer, K. Trinh, M. Nikkhoy and S. I. Bernstein (2007) Transcriptional regulation of the Drosophila melanogaster muscle myosin heavy-chain gene. Gene Expr. Patterns 7: 413-422.

Hao, Y., M. S. Miller, D. M. Swank, H. Liu, S. I. Bernstein, D. W. Maughan and G. H. Pollack (2006) Passive stiffness in Drosophila indirect flight muscle reduced by disrupting paramyosin phosphorylation, but not by embryonic myosin S2 hinge substitution. Biophys. J. 91: 4500-4506.

Miller, B. M. and S. I. Bernstein. (2006) Myosin. In Nature's Versatile Engine: Insect Flight Muscle Inside and Out. (J. Vigoreaux, ed.). Landes Biosciences, Georgetown TX. 62-75.

Swank, D. M., J. Braddock, W. Brown, H. Lesage, S. I. Bernstein and D. W. Maughan (2006) An alternative domain near the ATP binding pocket of Drosophila myosin affects muscle fiber kinetics. Biophys. J. 90; 2427-2435.

Melkani, G. C., A. Cammarato and S. I. Bernstein (2006) alphaB-Crystallin maintains skeletal muscle myosin enzymatic activity and prevents its aggregation under heat-shock stress. J. Mol. Biol. 358: 635-645. Journal Cover Image

Liu, H., M. S. Miller, D. M. Swank, W. A. Kronert, D. W. Maughan, and S. I. Bernstein. (2005) Paramyosin phosphorylation site disruption affects indirect flight muscle stiffness and power generation in Drosophila melanogaster. Proc. Natl. Acad. Sci. U.S.A. 102: 10522-10527.

Miller, B. M., S. Zhang, J. A. Suggs, D. M. Swank, K. P. Littlefield, A. F. Knowles and S.I. Bernstein. (2005) An alternative domain near the nucleotide-binding site of Drosophila muscle myosin affects ATPase kinetics. J. Mol. Biol. 353: 14-25.

Swank, D.M., W.A. Kronert, S.I. Bernstein and D.W. Maughan. (2004) Alternative N-terminal regions of Drosophila myosin heavy chain tune cross-bridge kinetics for optimal muscle power output. Biophys. J. 87: 1805-1814.

Hao, Y., S. I. Bernstein and G.H. Pollack. (2004) Passive stiffness of Drosophila IFM myofibrils: A novel, high accuracy measurement method. J. Muscle Res. Cell Motil. 25: 359-366.

Yu, Q. and S. I. Bernstein. (2003) UCS proteins: managing the myosin motor. Curr. Biol. 13: R525-R527.

Miller, B.M., M. Nyitrai, S. I. Bernstein, and M. A. Geeves. (2003). Kinetic analysis of Drosophila muscle myosin isoforms suggests a novel mode of mechanochemical coupling. J. Biol. Chem. 278: 50293-50300.

Swank, D. M., A. F. Knowles, W. A. Kronert, J. A. Suggs, G. Morrill, M. Nikkhoy, G. G. Manipon, and S. I. Bernstein. (2003) Variable N-terminal regions of muscle myosin heavy chain modulate ATPase rate and actin sliding velocity. J. Biol. Chem. 278: 17475-17482.

Littlefield, K. P., D. M. Swank, B. M. Sanchez, A. F. Knowles, D. M. Warshaw and S. I. Bernstein. (2003) The converter domain modulates the kinetic properties of Drosophila myosin. Am. J. Physiol. Cell Physiol. 284: C1031-C1038.

Swank, D. M., A. F. Knowles, J. A. Suggs, F. Sarsoza, A. Lee, D. W. Maughan and S. I. Bernstein. (2002) The myosin converter domain modulates muscle performance. Nature Cell Biol. 4: 312-317.

Arredondo, J.J., M. Mardahl-Dumesnil, R.M. Cripps, M. Cervera and S.I. Bernstein. (2001) Overexpression of miniparamyosin causes dysfunction and age-dependent myofibril degeneration in the indirect flight muscles of Drosophila melanogaster. J. Muscle Res. Cell Motil. 22: 287-299.

Swank, D.M., M.L. Bartoo, A.F. Knowles, C. Iliffe, S.I. Bernstein, J.E. Molloy and J.C. Sparrow. (2001) Alternative exon-encoded regions of Drosophila myosin heavy chain modulate ATPase rates and actin sliding velocity. J. Biol. Chem. 276: 15117-15124.

Zhang, S. and S.I. Bernstein. (2001). Spatially and temporally regulated expression of myosin heavy chain alternative exons during embryogenesis of Drosophila. Mech. Dev. 101: 35-39.

Arredondo, J.J., R.M. Ferreres, M. Maroto, R.M. Cripps, R. Marco, S. I. Bernstein and M. Cervera. (2001) Control of Drosophila paramyosin/miniparamyosin gene expression: differential regulatory mechanisms for muscle-specific transcription. J. Biol. Chem. 276: 8278-8287.

Cripps, R.M. and S.I. Bernstein. (2000) Generation of transgenic Drosophila melanogasterby P element-mediated germline transformation. In: Gene Transfer Methods: Introducing DNA into Living Cells and Organisms. (P.A. Norton and L.F. Steel, eds.), BioTechniques Books, Eaton Publishing. 93-125.

Swank, D.M., L. Wells, W.A. Kronert, G.E. Morrill and S.I. Bernstein. (2000) Determining structure/function relationships for sarcomeric myosin heavy chain by genetic and transgenic manipulation of Drosophila. Microsc. Res. Tech. 50: 430-442.

Cripps, R.M., J.A. Suggs and S.I. Bernstein. (1999) Assembly of thick filaments and myofibrils occurs in the absence of the myosin head. EMBO J. 18: 1793-1804.

Kronert, W.A., A. Acebes, A. Ferrus and S.I. Bernstein. (1999) Specific myosin heavy chain mutations suppress troponin I defects in Drosophila muscles. J. Cell Biol. 144: 989-1000.

Hodges, D., R.M. Cripps, M. O'Connor, and S.I. Bernstein. (1999) The role of evolutionarily-conserved sequences in alternative splicing at the 3' end of Drosophila melanogaster myosin heavy chain RNA. Genetics 151: 263-276.

Sharma, P. M. and S.I. Bernstein. (1997) Muscle, molecular genetics. In Encyclopedia of Human Biology. Academic Press. 6: 861-885.

Bernstein, S.I. and R.A. Milligan. (1997) Fine tuning a molecular motor: the location of alternative domains in the Drosophila myosin head. J. Mol. Biol. 271: 1-6. Journal Cover Image

Bernstein, S.I. and D. Hodges. (1997) mRNA splicing and alternative splicing. In: mRNA Metabolism and Post-transcriptional Gene Regulation. (J.B. Harford and D. Morris, eds). Wiley-Liss, New York. Modern Cell Biol. Series, 17: 43-60.

Wells, L., K.A. Edwards and S.I. Bernstein. (1996) Myosin heavy chain isoforms regulate muscle function but not myofibril assembly. EMBO J. 15: 4454-4459.

Kronert, W.A., P.T. O'Donnell, A. Fieck, A. Lawn, J.O. Vigoreaux, J.C. Sparrow and S.I. Bernstein. (1995) Defects in the Drosophila myosin rod permit sarcomere assembly but cause flight muscle degeneration. J. Mol. Biol. 249: 111-125.

Becker, K.D. and S.I. Bernstein. (1994) Genetic and transgenic approaches to dissecting muscle development and contractility using the Drosophila model system. Trends Cardiovasc. Med. 4: 243-250. Journal Cover Image

Fyrberg, E.A., S.I. Bernstein and K. VijayRaghavan. (1994) Basic methods for Drosophila muscle biology. In Drosophila melanogaster: Practical Uses in Cell and Molecular Biology. Methods Cell Biol. 44: 237-258.

Hodges, D. and S.I. Bernstein. (1994) Preparation and use of nuclear extracts from Drosophila cells for in vitro splicing. Methods Mol. Genet. 5: 91-108.

Cripps, R.M., K.D. Becker, M. Mardahl, W.A. Kronert, D. Hodges and S.I. Bernstein. (1994) Transformation of Drosophila melanogaster with the wild-type myosin heavy chain gene: rescue of mutant phenotypes and analysis of defects caused by overexpression. J. Cell Biol. 126: 689-699. Journal Cover Image

Hodges, D. and S.I. Bernstein. (1994) Genetic and biochemical approaches to dissecting the mechanism of alternative RNA splicing. Advances Genet. 31: 207-282.

Kronert, W.A., P.T. O'Donnell and S.I. Bernstein. (1994) A charge change in an evolutionarily-conserved region of the myosin globular head prevents myosin and thick filament accumulation in Drosophila. J. Mol. Biol. 236: 697-702.

Mardahl, M., R.M. Cripps, R.R. Rinehart, S.I. Bernstein and G.L. Harris (1993) Introduction of y+ onto a CyO chromosome. Drosophila Inform. Serv. 72: 141-142.

Bernstein, S.I., P.T. O'Donnell and R.M. Cripps. (1993) Molecular genetic analysis of muscle development, structure and function in Drosophila. Int. Rev. Cytol. 143: 63-152.

Hodges, D. and S.I. Bernstein. (1992) Suboptimal 5' and 3' splice sites regulate alternative splicing of Drosophila melanogaster myosin heavy chain transcripts in vitro. Mech. Dev. 37: 127-140.

Epstein, H.F. and S.I. Bernstein. (1992) Genetic approaches to understanding muscle development. Dev. Biol. 154: 231-244. Journal Cover Image

Becker, K.D., P.T. O'Donnell, J.M. Heitz, M. Vito and S.I. Bernstein. (1992) Analysis of Drosophila paramyosin: Identification of a novel isoform which is restricted to a subset of adult muscles. J. Cell Biol. 116: 669-681. Journal Cover Image

Kronert, W.A., K.A. Edwards, E.S. Roche, L. Wells and S.I. Bernstein. (1991) Muscle-specific accumulation of Drosophila myosin heavy chains: a splicing mutation in an alternative exon results in an isoform substitution. EMBO J. 10: 2479-2488.

Hess, N. and S.I. Bernstein. (1991) Developmentally-regulated alternative splicing of Drosophila myosin heavy chain transcripts: in vivo analysis of an unusual 3' splice site. Dev. Biol. 146: 339-344.

Collier, V.L., W.A. Kronert, P.T. O'Donnell, K.A. Edwards and S.I. Bernstein. (1990) Alternative myosin hinge regions are utilized in a tissue-specific fashion that correlates with muscle contraction speed. Genes & Dev. 4: 885-895. Journal Cover Image

O'Donnell, P.T., V.L. Collier, K. Mogami and S.I. Bernstein. (1989) Ultrastructural and molecular analyses of homozygous-viable Drosophila melanogaster muscle mutants indicate there is a complex pattern of myosin heavy chain isoform distribution. Genes & Dev. 3:1233-1246.

Zyskind, J.W. and S.I. Bernstein (1989) Recombinant DNA Laboratory Manual. Academic Press, San Diego. 195 pages. (1992) Revised edition. 224 pages.

Hess, N., W.A. Kronert and S.I. Bernstein (1989) Transcriptional and post-transcriptional regulation of Drosophila myosin heavy chain gene expression. In: Cellular and Molecular Biology of Muscle Development (L. Kedes and F. Stockdale, eds.) A.R. Liss, New York 621-631.

Johnson, G.G., W.A. Kronert, S.I. Bernstein, V.M. Chapman and K.D. Smith. (1988) Altered turnover of allelic variants of HPRT is associated with N-terminal amino acid sequence variation. J. Biol. Chem. 263:9079-9082.

O'Donnell, P.T. and S.I. Bernstein. (1988) Molecular and ultrastructural defects in a Drosophila myosin heavy chain mutant: differential effects on muscle function produced by similar thick filament abnormalities. J. Cell Biol. 107:2601-2612.

Emerson, C.P., Jr. and S.I. Bernstein (1987) Molecular genetics of myosin. Ann. Rev. Biochem. 56:695-726.

Wassenberg II, D.R., W.A. Kronert, P.T. O'Donnell and S.I. Bernstein. (1987) Analysis of the 5' end of the Drosophila muscle myosin heavy chain gene: alternatively spliced transcripts initiate at a single site and intron locations are conserved compared to myosin genes of other organisms. J. Biol. Chem. 262:10741-10747.

Bernstein, S.I., C.J. Hansen, K.D. Becker, D.R. Wassenberg, II, E.S. Roche, J.J. Donady and C.P. Emerson, Jr. (1986) Alternative RNA splicing generates transcripts encoding a thorax-specific isoform of Drosophila melanogaster myosin heavy chain. Mol. Cell. Biol. 6:2511-2519.

Mogami, K., P.T. O'Donnell, S.I. Bernstein, T.R.F. Wright and C.P. Emerson, Jr. (1986) Mutations of the Drosophila myosin heavy chain gene: effects on transcription, myosin accumulation, and muscle function. Proc. Natl. Acad. Sci. U.S.A. 83:1393-1397.

Bernstein, S.I., A.W. Glenn, C.P. Emerson, Jr., M.E. Minie and J.J. Donady. (1986) Purification of myosin heavy chain RNA from cultured Drosophila muscle cells and isolation of a myosin heavy chain gene. In: Techniques in the Life Sciences Volume C2: Techniques in In Vitro Invertebrate Hormones and Genes (E. Kurstak, ed.) Elsevier, Ireland

Bernstein, S.I., K. Mogami, J.J. Donady, and C.P. Emerson, Jr., (l983) Drosophila muscle myosin heavy chain is encoded by a single gene located in a cluster of muscle mutations. Nature 302:393-397.